多巴色素異構(gòu)酶(DT)重組蛋白
適用生物    Homo sapiens (Human，人)
多巴色素異構(gòu)酶(DT)重組蛋白
產(chǎn)品應(yīng)用    SDS-PAGE; WB; ELISA; IP.    規(guī)格    50ug
分子量    30.9kDa    價格    請咨詢當?shù)卮砩?/span>
純度    > 95%   
來源    原核表達    

Recombinant Dopachrome Tautomerase (DCT)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Homo sapiens (Human)
Product No.	HZB066Hu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	30.9kDa
Predicted isoelectric point	8.5
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1μg (determined by the LAL method)

Subcellular Location	Melanosome membrane; Single-pass type I membrane protein.
Residues	Val28~Gly263 (Accession # P40126) with two N-terminal Tags, His-tag and T7-tag
Formulation	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES

多巴色素異構(gòu)酶(DT)重組蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VCM TVDSLVNKEC CPRLGAESAN VCGSQQGRGQ CTEVRADTRP WSGPYILRNQ DDRELWPRKF FHRTCKCTGN FAGYNCGDCK FGWTGPNCER KKPPVIRQNI HSLSPQEREQ FLGALDLAKK RVHPDYVITT QHWLGLLGPN GTQPQFANCS VYDFFVWLHY YSVRDTLLGP GRPYRAIDFS HQGPAFVTWH RYHLLCLERD LQRLIGNESF ALPYWNFATG RNECDVCTDQ LFG

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

多巴色素異構(gòu)酶(DT)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

多巴色素異構(gòu)酶(DT)重組蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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