分子量 63.6kDa 價格 請咨詢當(dāng)?shù)卮砩?/span>
純度 > 95%
來源 原核表達(dá)
Recombinant Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Rattus norvegicus (Rat)
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Product No.
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HZB134Ra02
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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63.6kDa
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Predicted isoelectric point
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n/a
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1μg (determined by the LAL method)
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Subcellular Location
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n/a
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Residues
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Gly1919~Lys2258 (Accession # Q00657) with two N-terminal Tags, His-tag and GST-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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SEQUENCES
黑色素瘤關(guān)聯(lián)硫酸軟骨素蛋白聚糖(MCSP)重組蛋白The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-GV FQLSMSDGAS PPIPMSLAVD VLPSTIEVQL RAPLEVPQAL GRSSLSRQQL QVISDREEPD VAYRLTQGPL YGQVLVGGQP ASAFSQLQVD QGDVVFAFTN FSSSQDHFKV LALARGVNAS ATVNVTVQAL LHVWAGGPWP QGTTLRLDPT VLDASELANR TGSMPRFRLL EGPRYGRVVR VSQGRAESRT NQLVEDFTQQ DLEEGRLGLE VGRPEGRSTG PTGDRLTLEL QATGVPPAVA LLDFATEPYH AAKFYKVTLL SVPEAARTET EKTGKSTPTG QPGQAASSPM PTVAKSGFLG FLEANMFSVI IPVCLVLLLL ALILPLLFYL RKRNKTGK
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
黑色素瘤關(guān)聯(lián)硫酸軟骨素蛋白聚糖(MCSP)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
黑色素瘤關(guān)聯(lián)硫酸軟骨素蛋白聚糖(MCSP)重組蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
