分子量 57.9kDa 價格 請咨詢當(dāng)?shù)卮砩?/span>
純度 > 95%
來源 原核表達(dá)
Recombinant Bruton'S Tyrosine Kinase (Btk)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Homo sapiens (Human)
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Product No.
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HZB915Hu01
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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57.9kDa
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Predicted isoelectric point
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n/a
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1μg (determined by the LAL method)
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Subcellular Location
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n/a
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Residues
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Glu396~Ser659 (Accession # Q06187) with two N-terminal Tags, His-tag and GST-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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SEQUENCES
布魯東氏酪氨酸激酶(Btk)重組蛋白The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- EIDPK DLTFLKELGT GQFGVVKYGK WRGQYDVAIK MIKEGSMSED EFIEEAKVMM NLSHEKLVQL YGVCTKQRPI FIITEYMANG CLLNYLREMR HRFQTQQLLE MCKDVCEAME YLESKQFLHR DLAARNCLVN DQGVVKVSDF GLSRYVLDDE YTSSVGSKFP VRWSPPEVLM YSKFSSKSDI WAFGVLMWEI YSLGKMPYER FTNSETAEHI AQGLRLYRPH LASEKVYTIM YSCWHEKADE RPTFKILLSN ILDVMDEES
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
布魯東氏酪氨酸激酶(Btk)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
布魯東氏酪氨酸激酶(Btk)重組蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-12155R phospho-Cyclin D3 (Thr283)磷酸化周期素D3抗體 濃度1mg/ml
yb-12162R CYB5R3細(xì)胞色素b5還原酶3抗體 濃度1mg/ml
yb-11464R CWC22CWC22蛋白抗體 濃度1mg/ml
yb-15349R C9orf959號染色體開放閱讀框95抗體 濃度1mg/ml
yb-13794R C19orf6719號染色體開放閱讀框67抗體 濃度1mg/ml
yb-12426R phospho-Casein kinase I isoforms gamma 1+2+3 ( Tyr263)磷酸化酪蛋白激酶1γ1+γ2+γ3抗體 濃度1mg/ml
yb-8860R Coproporphyrinogen III Oxidase原卟啉氧化酶3抗體 濃度1mg/ml
yb-8987R Connexin 43間隙連接蛋白43抗體 濃度1mg/ml
yb-13745R CLDN25緊密連接蛋白25抗體 濃度1mg/ml
yb-13863R CEP78中心體蛋白78抗體 濃度1mg/ml
yb-15223R C6orf1466號染色體開放閱讀框146抗體 濃度1mg/ml
yb-15226R C6ORF1546號染色體開放閱讀框154抗體 濃度1mg/ml
yb-15316R C9orf169號染色體開放閱讀框16抗體 濃度1mg/ml
yb-15342R C9orf789號染色體開放閱讀框78抗體 濃度1mg/ml
yb-15343R C9orf79 9號染色體開放閱讀框79抗體 濃度1mg/ml
yb-15344R C9orf849號染色體開放閱讀框84抗體 濃度1mg/ml
yb-15345R C9orf859號染色體開放閱讀框85抗體 濃度1mg/ml
yb-15347R C9ORF919號染色體開放閱讀框91抗體 濃度1mg/ml
yb-15348R C9orf939號染色體開放閱讀框93抗體 濃度1mg/ml
yb-15350R C9orf969號染色體開放閱讀框96抗體 濃度1mg/ml