載脂蛋白B(APOB)重組蛋白
適用生物    Homo sapiens (Human，人)
載脂蛋白B(APOB)重組蛋白
產(chǎn)品應(yīng)用    SDS-PAGE; WB; ELISA; IP.    規(guī)格    50ug
分子量    21.4kDa    價格    請咨詢當(dāng)?shù)卮砩?/span>
純度    > 95%    
來源    原核表達(dá)

Recombinant Apolipoprotein B (APOB)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Homo sapiens (Human)
Product No.	HZC003Hu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	21.4kDa
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1μg (determined by the LAL method)
Residues	His3365~Glu3548 with N-terminal His-Tag

Formulation

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative

SEQUENCES

載脂蛋白B(APOB)重組蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
HLLSSS SSVIDALQYK LEGTTRLTRK RGLKLATALS LSNKFVEGSH NSTVSLTTKN MEVSVATTTK AQIPILRMNF KQELNGNTKS KPTVSSSMEF KYDFNSSMLY STAKGAVDHK LSLESLTSYF SIESSTKGDV KGSVLSREYS GTIASEANTY LNSKSTRSSV KLQGTSKIDD IWNLEVKE

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

載脂蛋白B(APOB)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

載脂蛋白B(APOB)重組蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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