產(chǎn)品描述
GAS-GFP報(bào)告基因是自主研發(fā)的用于檢測STAT1/STAT1轉(zhuǎn)錄活性水平為目的的報(bào)告基因。GAS(gamma-activated sequence, GAS) 又稱γ干擾素活化序列在皮膚發(fā)生遲發(fā)型接觸性過敏時(shí)，可與轉(zhuǎn)位至角朊細(xì)胞核內(nèi)的STAT-91結(jié)合而激活K17的表達(dá)。
GAS-GFP報(bào)告基因主要用于檢測細(xì)胞中干擾素調(diào)控的信號(hào)通路的活性、**研究以及基因過表達(dá)和RNAi的表型分析等。
pGMGAS-GFP是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)STAT1/STAT1結(jié)合位點(diǎn)，可以高效地檢測STAT1/STAT1的激活水平。由于載體采用了GFP作為報(bào)告基因，更便于后續(xù)的檢測。同時(shí)，對(duì)載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得GAS-GFP報(bào)告基因更易于轉(zhuǎn)染。GAS GFP Reporter Plasmid（GAS-GFP報(bào)告基因質(zhì)粒）
質(zhì)粒圖譜
GAS GFP Reporter Plasmid（GAS-GFP報(bào)告基因質(zhì)粒）pGM GAS -GFP質(zhì)粒測序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃保存。
使用說明
pGMGAS-GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。
注意事項(xiàng)
1）本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
2）為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。GAS GFP Reporter Plasmid（GAS-GFP報(bào)告基因質(zhì)粒）
參考文獻(xiàn)
[1] Wu AM, et al. Induction of Multidrug Resistance Transporter ABCG2 by Prolactin in Human Breast Cancer Cells.Mol Pharmacol. 83(2):377-88 (2013).
[2] Jung MR,et al. Suppression of thymus- and activation-regulated chemokine (TARC/CCL17) production by 3-O-β-D-glucopyanosylspinasterol via blocking NF-κB and STAT1 signaling pathways in TNF-α and IFN-γ-induced HaCaT keratinocytes. Biochem Biophys Res Commun. 427(2):236-41(2012).
[3] Kim YJ, et al. C6 glioma cell insoluble matrix components enhance interferon-gamma- stimulated inducible nitric-oxide synthase/nitric oxide production in BV2 microglial cells. J Biol Chem. 283(5):2526-33(2008).
[4] Ahmed CM, Johnson HM. IFN-gamma and its receptor subunit IFNGR1 are recruited to the IFN-gamma-activated sequence element at the promoter site of IFN-gamma-activated genes: evidence of transactivational activity in IFNGR1. J Immunol. 177(1):315-21(2006).
[5] Osaki M,et al.The TATA-containing core promoter of the type II collagen gene (COL2A1) is the target of interferon-gamma-mediated inhibition in human chondrocytes: requirement for Stat1 alpha, Jak1 and Jak2.Biochem J. 369(Pt 1):103-15 (2003).GAS GFP Reporter Plasmid（GAS-GFP報(bào)告基因質(zhì)粒）
