產(chǎn)品描述
IL6-Promoter熒光素酶報(bào)告基因（報(bào)告基因質(zhì)粒）（IL6-Promoter luciferase reporter plasimd）是自主研發(fā)的用于檢測(cè)human IL6-Promoter激活水平為目的的報(bào)告基因。IL6(Interleukin 6)白細(xì)胞介素-6，簡(jiǎn)稱白介素6，是一種重要的細(xì)胞炎性細(xì)胞因子，在炎性**的發(fā)**展中起了重要的作用，常作為炎性反應(yīng)的重要指標(biāo)。
IL6-Promoter報(bào)告基因主要應(yīng)用于Jak/Stat信號(hào)通路的研究等和human IL6-Promoter激活水平的檢測(cè)等。
pGMIL6-Promoter-Lu是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了human IL6啟動(dòng)子，可以高靈敏度地檢測(cè)human IL6-Promoter的激活水平。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒對(duì)轉(zhuǎn)錄因子結(jié)合的特異性。另外，由于質(zhì)粒體積減小，使得IL6-Promoter報(bào)告基因質(zhì)粒更易于轉(zhuǎn)染。IL6-promoter luciferase reporter plasmid（IL6-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）
質(zhì)粒圖譜
IL6-promoter luciferase reporter plasmid（IL6-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）運(yùn)輸與保存方法
冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20 oC保存。
注意事項(xiàng)
本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。
使用說明
pGMIL6-promoter-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
參考文獻(xiàn)IL6-promoter luciferase reporter plasmid（IL6-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）
[1] OHara SP, et al. Cholangiocyte N-Ras protein mediates lipopolysaccharide-induced interleukin 6 secretion and proliferation. J Biol Chem. 286(35):30352-60 (2011).
[2] Xiao W, et al. Co-operative functions between nuclear factors NFkappaB and CCAT/enhancer-binding protein-beta (C/EBP-beta) regulate the IL-6 promoter in autocrine human prostate cancer cells. Prostate. 61(4):354-70 (2004).
[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).
[7] Tran TA, et al. Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor- kappa B. PLoS One. 6(8):e23660 (2011).
[8] Robbins D, et al. Imaging NF-κB signaling in mice for screening anticancer drugs. Methods Mol Biol. 716: 169- 77 (2011).
[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells. ChemBiol Interact. 159(2):117-28 (2006).
[10] Ozeki M, et al. Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD. Anticancer Res. 24(5A):2657-63 (2004).IL6-promoter luciferase reporter plasmid（IL6-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）
