產(chǎn)品描述
MEF1-Luc熒光素酶報(bào)告基因質(zhì)粒（MEF1 luciferase reporter plasmid）是自主研發(fā)的用于檢測(cè)MEF1(myocyte enhancer factor 1)轉(zhuǎn)錄活性水平為目的的報(bào)告基因。
MEF1-Luc熒光素酶報(bào)告基因質(zhì)粒主要應(yīng)用于Myocyte Enhancer Factor 1信號(hào)通路、**研究、相關(guān)基因的調(diào)控和功能的研究。
pGMMEF1-Lu是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)MEF1結(jié)合位點(diǎn)，可以高靈敏度地檢測(cè)MEF1的激活水平。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得MEF1報(bào)告基因質(zhì)粒更易于轉(zhuǎn)染。MEF1 luciferase reporter plasmid MEF1-Luc熒光素酶報(bào)告基因質(zhì)粒
質(zhì)粒圖譜
MEF1 luciferase reporter plasmid MEF1-Luc熒光素酶報(bào)告基因質(zhì)粒運(yùn)輸與保存方法
冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20 oC保存。
使用說(shuō)明
pGMMEF1-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
注意事項(xiàng)
本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。MEF1 luciferase reporter plasmid MEF1-Luc熒光素酶報(bào)告基因質(zhì)粒
參考文獻(xiàn)
[1] Shareef MM, et al. Lack of P-glycoprotein expression by low-dose fractionated radiation results from loss of nuclear factor-kappaB and NF-Y activation in oral carcinoma cells. Mol Cancer Res. 6(1):89-98(2008).
[2] Falcone G, et al. v-Src inhibits myogenic differentiation by interfering with the regulatory network of muscle-specific transcriptional activators at multiple levels. Oncogene. 22(51):8302-15 (2003).
[3] Ogretmen B, et al. Identification and characterization of the MDR1 promoter-enhancing factor 1 (MEF1) in the multidrug resistant HL60/VCR human acute myeloid leukemia cell line. Biochemistry. 39(1):194-204 (2000).MEF1 luciferase reporter plasmid MEF1-Luc熒光素酶報(bào)告基因質(zhì)粒
