STAT3 luciferase reporter plasmid （STAT3螢火蟲熒光素酶報(bào)告基因質(zhì)粒）運(yùn)輸與保存方法

冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20 oC保存。

注意事項(xiàng)

本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。

為了您的健康，實(shí)驗(yàn)操作時(shí)請穿實(shí)驗(yàn)服和帶一次性手套。

使用說明

pGMSTAT3-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測試劑盒或雙熒光素酶檢測試劑盒進(jìn)行檢測。

參考文獻(xiàn)

[1] Kang Y, et al. The radio-sensitizing effect of xanthohumol is mediated by STAT3 and EGFR suppression in doxorubicin-resistant MCF-7 human breast cancer cells. Biochim Biophys Acta. 1830(3):2638-48 (2013).STAT3 luciferase reporter plasmid （STAT3螢火蟲熒光素酶報(bào)告基因質(zhì)粒）

[2] Teng J, et al. HBx-dependent activation of Twist mediates STAT3 Control of epithelium-mesenchymal transition of liver cells. J Cell Biochem. 114(5):1097-104 (2013).

[3] Villa S, et al. The influence of the substitution pattern on the molecular conformation of ureido-1,2,5-oxadiazoles, related to STAT3 inhibitors: chemical behavior and structural investigation. ChemBiodivers. 9(7):1240-53 (2012).

[4] Han L, et al. MicroRNA-21 expression is regulated by β-catenin/STAT3 pathway and promotes glioma cell invasion by direct targeting RECK. CNS NeurosciTher. 18(7):573-83 (2012).

[5] Kwon MJ, et al. Protein kinase C-θ promotes Th17 differentiation via upregulation of Stat3. J Immunol. 188(12):5887-97 (2012).

[6] Seo HS, et al. Apigenin induces apoptosis via extrinsic pathway, inducing p53 and inhibiting STAT3 and NFκB signaling in HER2-overexpressing breast cancer cells. Mol Cell Biochem. 366(1-2):319-34 (2012).

[7] Lim EJ, et al. Methylsulfonylmethane suppresses breast cancer growth by down-regulating STAT3 and STAT5b pathways. PLoS One. 7(4):e33361 (2012).

[8] Hahm ER, et al. Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cells. Cancer Prev Res (Phila). 3(4):484-94 (2010).

[9] Lin L, et al. A novel small molecule, LLL12, inhibits STAT3 phosphorylation and activities and exhibits potent growth-suppressive activity in human cancer cells. Neoplasia. 12(1):39-50 (2010).STAT3 luciferase reporter plasmid （STAT3螢火蟲熒光素酶報(bào)告基因質(zhì)粒）

[10] Shin DS, et al. Cryptotanshinone inhibits constitutive signal transducer and activator of transcription 3 function through blocking the dimerization in DU145 prostate cancer cells. Cancer Res. 69(1):193-202 (2009).

[11] Duan Z, et al. Signal transducers and activators of transcription 3 pathway activation in drug-resistant ovarian cancer. Clin Cancer Res. 12(17):5055-63