Polyclonal Antibody to Aprotinin (AP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: Aprotinin, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Native aprotinin of human. Accession No.: NPA968Hu01
[ REFERENCES ] 抑肽酶(AP)多克隆抗體The aprotinin is the small protein pancreatic trypsin inhibitor, or BPTI, which
inhibits trypsin and related proteolytic enzymes. Aprotinin was used as a
medication administered by injection to reduce bleeding during complex surgery,
such as heart and liver surgery. In cardiac surgery with a high risk of significant
blood loss, aprotinin significantly reduced bleeding, mortality and hospital stay.
Beneficial effects were also reported in high-risk orthopedic surgery. In liver
transplantation, initial reports of benefit were overshadowed by concerns about
toxicity.
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against aprotinin. It has been
selected for its ability to recognize aprotinin in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.抑肽酶(AP)多克隆抗體 [ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.