Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: BNP, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant BNP (His27~His134) with N-terminal His-Tag
expressed in E.coli. Accession No.: RPA541Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against BNP. It has been
selected for its ability to recognize BNP in immunohistochemical staining and
western blotting.腦鈉素(BNP)多克隆抗體 [ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant BNP (His27~His134) disposed
in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 腦鈉素(BNP)多克隆抗體[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant BNP, Human
Used in DAB staining on fromalin fixed
paraffin- embedded liver tissue
PAA541Hu01
Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in DAB staining on fromalin fixed
paraffin- embedded brain tissue
Used in DAB staining on fromalin fixed
paraffin- embedded liver cancer tissue
Used in DAB staining on fromalin fixed
paraffin- embedded breast cancer tissue
Used in DAB staining on fromalin fixed
paraffin- embedded lung cancer tissue
PAA541Hu01
Polyclonal Antibody to Brain Natriuretic Peptide (BNP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in DAB staining on fromalin fixed paraffinembedded
prostate glands cancer tissue
Used in DAB staining on fromalin fixed
paraffin- embedded prostate glands tissue
Used in DAB staining on fromalin fixed
paraffin- embedded rectum cancer tissue
Used in DAB staining on fromalin fixed
paraffin- embedded pancreas cancer tissue