視黃醇結(jié)合蛋白3(RBP3)多克隆抗體
PAA367Hu01 Polyclonal Antibody to Retinol Binding Protein 3, Interstitial (RBP3) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: RBP3, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg Sample: Recombinant RBP3, Human [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant RBP3 (Pro19~Leu320) expressed in E.coli. Accession No.: RPA367Hu01 Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-PT HLFQPSLVLD MAKVLLDNYC FPENLLGMQE AIQQAIKSHE ILSISDPQTL ASVLTAGVQS SLNDPRLVIS YEPSTPEPPP QVPALTSLSE EELLAWLQRG LR HE V LE G NV GY L RV DS V P G QE V L SMMG EF LVA HVW G N LM GTS A LV L D L R HCTGGQVSGI PYIISYLHPG NTILHVDTIY NRPSNTTTEI WTLPQVLGER YGADKDVVVL TSSQTRGVAE DIAHILKQMR RAIVVGERTG GGALDLRKLR IGESDFFFTV PVSRSLGPLG GGSQTWEGSG VLPCVGTPAE QALEKALAIL [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against RBP3. 視黃醇結(jié)合蛋白3(RBP3)多克隆抗體It has been selected for its ability to recognize RBP3 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant RBP3 (Pro19~Leu320) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.視黃醇結(jié)合蛋白3(RBP3)多克隆抗體