腫瘤蛋白p53(TP53)多克隆抗體

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物無標記物
• **原腫瘤蛋白p53(TP53)重組蛋白
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用腫瘤蛋白p53(TP53)重組蛋白WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
• Polyclonal Antibody to Tumor Protein p53 (TP53) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Polyclonal antibody preparation Host: Rabbit Purification: Antigen-specific Affinity Chromatography. Traits: Liquid Concentration: 200μg/mL UOM: 100μg Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ] Immunogen: Recombinant TP53 (Gly108~Lys370) expressed in E.coli. Accession No.: RPA928Hu01 [ APPLICATIONS ] Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. [ FORMULATION ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. 腫瘤蛋白p53(TP53)重組蛋白[ QUALITY CONTROL ] Content: The quality control contains recombinant TP53 disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 4 oC for frequent use. Aliquot and store at -20 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ IDENTIFICATION ] A B Figure 1. Western Blot A. Sample: Recombinant TP53, Human B. Human MCF7 Cells Primary Ab: 2μg/mL Rabbit Anti-Human TP53 Ab Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SAA544Rb59)腫瘤蛋白p53(TP53)重組蛋白