甲胎蛋白(αFP)多克隆抗體

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物甲胎蛋白(αFP)多克隆抗體無(wú)標(biāo)記物
• **原RPA153Hu01-甲胎蛋白(aFP)重組蛋白
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用甲胎蛋白(αFP)多克隆抗體WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
• Polyclonal Antibody to Alpha-Fetoprotein (aFP) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Polyclonal antibody preparation Host: Rabbit Purification: Antigen-specific Affinity Chromatography. Traits: Liquid Concentration: 200μg/mL UOM: 100μg Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ] Immunogen: RPA153Hu01-Recombinant Alpha-Fetoprotein (aFP) [ APPLICATIONS ] Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. 甲胎蛋白(αFP)多克隆抗體[ FORMULATION ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant aFP disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 4 oC for frequent use. Aliquot and store at -20 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ IDENTIFICATION ] A B Figure 1. Western Blot A. Sample: Recombinant aFP, Human B. Lane1: Human Liver Tissue Lane2: Human Lung Tissue Primary Ab: 3μg/mL Rabbit Anti-Human aFP Ab Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SAA544Rb59) A Figure 2. DAB staining on IHC-P Samples： A. Human Liver Tissue B. Human Liver Cancer Tissue C. Human Stomach Tissue C B甲胎蛋白(αFP)多克隆抗體