來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物色氨酸(Trp)多克隆抗體無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用色氨酸(Trp)多克隆抗體WB, ICC, IHC-P, IHC-F, ELISA
A90780Hu01 90780Hu01 90780Hu01 90780Hu01 Polyclonal Antibody Antibody Antibody Antibody to Lactoferrin Lactoferrin Lactoferrin Lactoferrin (LTF) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 5th Edition (Revised in January, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: LTF Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Species Species Species Species Reactivity: eactivity: eactivity: eactivity: Human Ig type: Rabbit IgG Purification: Purification: Purification: Purification: Antibodies are purified by target protein affinity chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F Form: Liquid Size: 100μg Sample: Recombinant human LTF [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant human LTF 色氨酸(Trp)多克隆抗體(Glu542~Gln685) expressed in E.coli. Molecular Molecular Molecular Molecular Weight: Weight: Weight: Weight: 17.8kDa USCN accession accession accession accession No.: P90780Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF- ERYYGYTGA FRCLAENAGD VAFVKDVTVL QNTDGNNNEA WAKDLKLADF ALLCLDGKRK PVTEARSCHL AMAPNHAVVS RMDKVERLKQ VLLHQQAKFG RNGSDCPDKF CLFQSETKNL LFNDNTECLA RLHGKTTYEK YLGPQ [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPEFICITY SPEFICITY SPEFICITY SPEFICITY ] Anti LTF is a rabbit polyclonal antibody raised against human LTF. This antibody has been selected for its ability to recognize human LTF in immunohistochemical staining and western blotting, non cross-reactive with other members of the family. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] 色氨酸(Trp)多克隆抗體Content: Content: Content: Content: The quality control contains recombinant human LTF (Glu542~Gln685) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading buffer: buffer: buffer: buffer: 100mM Tris(PH 8.8), 2%SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, Na3N 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.