來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
**原顆粒酶H(GZMH)多克隆抗體-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用顆粒酶H(GZMH)多克隆抗體WB, ICC, IHC-P, IHC-F, ELISA
PAL575Hu01 Polyclonal Antibody to Granzyme H (GZMH) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: GZMH, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant GZMH (Glu19~Leu246) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPL575Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against GZMH. It has been selected for its ability to recognize GZMH in immunohistochemical staining and western blotting. 顆粒酶H(GZMH)多克隆抗體[ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant GZMH (Glu19~Leu246) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] 顆粒酶H(GZMH)多克隆抗體Used in Western Blot, Sample: Recombinant GZMH, Human Used in DAB staining on fromalin fixed paraffin- embedded spleen tissue PAL575Hu01 Polyclonal Antibody to Granzyme H (GZMH) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in Western Blot, Sample: Jurkat Cells Primary Ab: 2μg/mL Rabbit Anti-Human GZMH Ab Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SECab05)