來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物蛋白酶激活受體4(PAR4)多克隆抗體無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
蛋白酶激活受體4(PAR4)多克隆抗體Polyclonal Antibody to Protease Activated Receptor 4 (PAR4) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: PAR4, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant PAR4 (Gly48~Gln385) with two N-terminal Tags, His-tag and GST-tag expressed in E.coli. Accession No.: RPA127Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against PAR4. It has been selected for its ability to recognize PAR4 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.蛋白酶激活受體4(PAR4)多克隆抗體 [ QUALITY CONTROL ] Content: The quality control contains recombinant PAR4 (Gly48~Gln385) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant PAR4, Human Used in DAB staining on fromalin fixed paraffin- embedded testis tissue 】PAA127Hu01 Polyclonal Antibody to Protease Activated Receptor 4 (PAR4) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in Western Blot, Sample: Hela Cells Primary Ab: 3μg/mL Rabbit Anti-Human PAR4 Ab Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SECab05)蛋白酶激活受體4(PAR4)多克隆抗體