來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物組織金屬蛋白酶抑制因子3(TIMP3)多克隆抗體無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
組織金屬蛋白酶抑制因子3(TIMP3)多克隆抗體Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Organism Species: Bos taurus; Bovine (Cattle) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: TIMP3, Bovine Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg Sample: Recombinant TIMP3, Bovine [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant TIMP3 (Pro31~Thr209) expressed in E.coli. Accession No.: RPA129Bo01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PQDAFCNSDI VIRAKVVGKK LLKEGPFGTM VYTIKQMKMY RGFTKMPHVQ YIHTEASESL CGLKLEVNKY QYLLTGRVYD GKMYTGLCNF VERWDQLTLS QRKGLNYRYH LGCNCKIKSC YYLPCFVTSK NECLWTDMFS NFGYPGYQSK HYACIRQKGG YCSWYRGWAP PDKSIINAT [ ANTIBODY SPECIFITY ]組織金屬蛋白酶抑制因子3(TIMP3)多克隆抗體 The antibody is a rabbit polyclonal antibody raised against TIMP3. It has been selected for its ability to recognize TIMP3 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant TIMP3 (Pro31~Thr209) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.組織金屬蛋白酶抑制因子3(TIMP3)多克隆抗體 [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.