心鈉肽(ANP)多克隆抗體 Polyclonal Antibody to Atrial Natriuretic Peptide (ANP) Organism Species: Bos taurus (Bovine) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: ANP, Bovine Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg Sample: Recombinant ANP, Bovine [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant ANP (Asn25~Arg152) expressed in E.coli. Accession No.: RPA225Bo01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-NPVYGS VS N A D LM D F K N L L D R L E D K M P L E D E AV P S Q V L S E Q N E E A G AP L S P L S E M P PWMGE V NPA Q RE G G V L G R GP WE S S D RS A L L KS K L RA L LTA PRS L R RSS C F GGRMDRIGAQ SGLGCNSFRY RR [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against ANP. It has been selected for its ability to recognize ANP in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. 心鈉肽(ANP)多克隆抗體[ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant ANP (Asn25~Arg152) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.心鈉肽(ANP)多克隆抗體

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