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產(chǎn)品名稱: 蛋白激酶R(PKR)多克隆抗體
產(chǎn)品型號: 蛋白激酶R多克隆抗體
產(chǎn)品展商: Abcam
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
蛋白激酶R(PKR)多克隆抗體是針對PKR的兔多克隆抗體。在**組織化學(xué)染色和**印跡實驗中能識別PKR。蛋白激酶R(PKR)多克隆抗體豐富產(chǎn)品組合，其中包括超過 42,000 多種高品質(zhì)抗體，并配套提供種類齊全的抗體相關(guān)產(chǎn)品。 我們的抗體測定結(jié)果經(jīng)全球上千次的引用驗證，有助于您在一系列抗體應(yīng)用中獲得優(yōu)異的實驗結(jié)果。
蛋白激酶R(PKR)多克隆抗體
的詳細(xì)介紹
蛋白激酶R(PKR)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物蛋白激酶R(PKR)多克隆抗體無標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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蛋白激酶R(PKR)多克隆抗體Polyclonal Antibody to Protein Kinase R (PKR)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: PKR, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant PKR (Ser224~Ile502) expressed in E.coli. Accession No.: RPA520Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SSSLLMN GLRNNQRKAK
RSLAPRFDLP DMKETKYTVD KRFGMDFKEI ELIGSGGFGQ VFKAKHRIDG KTYVIKRVKY
NNEKAEREVK ALAKLDHVNI VHYNGCWDGF DYDPETSDDS LESSDYDPEN SKNSSRSKTK
CLFIQMEFCD KGTLEQWIEK RRGEKLDKVL ALELFEQITK GVDYIHSKKL IHRDLKPSNI
FLVDTKQVKI GDFGLVTSLK NDGKRTRSKG TLRYMSPEQI SSQDYGKEVD LYALGLILAE
LLHVCDTAFE TSKFFTDLRD GI
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against PKR. It has been
selected for its ability to recognize PKR in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. 蛋白激酶R(PKR)多克隆抗體[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant PKR (Ser224~Ile502)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Brain tissue
Used in Western Blot, Sample:
Recombinant PKR蛋白激酶R(PKR)多克隆抗體