神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體
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產(chǎn)品名稱: 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體
產(chǎn)品型號: NSE
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體是針對NSE的兔多克隆抗體。在**組織化學(xué)染色和**印跡實驗中能識別NSE。神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體豐富產(chǎn)品組合，其中包括超過 42,000 多種高品質(zhì)抗體，并配套提供種類齊全的抗體相關(guān)產(chǎn)品。 我們的抗體測定結(jié)果經(jīng)全球上千次的引用驗證，有助于您在一系列抗體應(yīng)用中獲得優(yōu)異的實驗結(jié)果。
神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體
的詳細介紹
神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體無標(biāo)記物
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**原神經(jīng)元特異性烯醇化酶(NSE)重組蛋白
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體Polyclonal Antibody to Enolase, Neuron Specific (NSE)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200μg/mL
UOM: 100μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPA537Hu01-Recombinant Enolase, Neuron Specific (NSE)
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A
A B
Figure 1. Western Blot
A. Sample: Recombinant NSE, Human B. Lane1: Mouse Muscle Tissue Lane2: Human Mcf7 Cells Lane3: Human A549 Cells Primary Ab: 2μg/mL Rabbit Anti-Human NSE Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)
Figure 2. DAB staining on
IHC-P
Samples：
A. Human Liver Tissue神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體