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產(chǎn)品名稱: 糖原合酶激酶3α(GSK3α)多克隆抗體
產(chǎn)品型號: GSK3α
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
糖原合酶激酶3α(GSK3α)多克隆抗體是針對GSK3a的兔多克隆抗體。在**組織化學(xué)染色和**印跡實驗中能識別GSK3a。糖原合酶激酶3α(GSK3α)多克隆抗體豐富產(chǎn)品組合，其中包括超過 42,000 多種高品質(zhì)抗體，并配套提供種類齊全的抗體相關(guān)產(chǎn)品。 我們的抗體測定結(jié)果經(jīng)全球上千次的引用驗證，有助于您在一系列抗體應(yīng)用中獲得優(yōu)異的實驗結(jié)果。
糖原合酶激酶3α(GSK3α)多克隆抗體
的詳細介紹
糖原合酶激酶3α(GSK3α)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物糖原合酶激酶3α(GSK3α)多克隆抗體無標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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糖原合酶激酶3α(GSK3α)多克隆抗體Polyclonal Antibody to Glycogen Synthase Kinase 3 Alpha (GSK3a)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: GSK3a, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 50μg Sample: Recombinant GSK3a, Human
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant GSK3a (Gln115~Leu409) expressed in E.coli. Accession No.: RPA630Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- QEVAYT DIKVIGNGSF
GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY SSGEKKDELY
LNLVLEYVPE TVYRVARHFT KAKLTIPILY VKVYMYQLFR SLAYIHSQGV CHRDIKPQNL
LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS IDVWSAGCVL
AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK AHPWTKVFKS
RTPPEAIALC SSLLEYTPSS RLSPLEACAH SFFDELRCL糖原合酶激酶3α(GSK3α)多克隆抗體 [ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against GSK3a. It has been
selected for its ability to recognize GSK3a in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant GSK3a (Gln115~Leu409)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. 糖原合酶激酶3α(GSK3α)多克隆抗體Avoid repeated freeze-thaw cycles.
