脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體Polyclonal Antibody to Phospholipase A2, Lipoprotein Associated (LpPLA2) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: LpPLA2, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant LpPLA2 (Phe22~Asn441) expressed in E.coli. Accession No.: RPA867Hu01 Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEFELRRQ-FDWQYINPV AHMKSSAWVN KIQVLMAAAS FGQTKIPRGN GPYSVGCTDL MFDHTNKGTF LRLYYPSQDN DRLDTLWIPN KEYFWGLSKF LGTHWLMGNI LRLLFGSMTT PANWNSPLRP GEKYPLVVFS HGLGAFRTLY SAIGIDLASH GFIVAAVEHR DRSASATYYF KDQSAAEIGD KSWLYLRTLK QEEETHIRNE QVRQRAKECS QALSLILDID HGKPVKNALD LKFDMEQLKD SIDREKIAVI GHSFGGATVI QTLSEDQRFR CGIALDAWMF PLGDEVYSRI PQPLFFINSE YFQYPANIIK MKKCYSPDKE RKMITIRGSV HQNFADFTFA TGKIIGHMLK LKGDIDSNVA IDLSNKASLA FLQKHLGLHK DFDQWDCLIE GDDENLIPGT NINTTNQHIM LQNSSGIEKY N [ ANTIBODY SPECIFITY ] 脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體The antibody is a rabbit polyclonal antibody raised against LpPLA2. It has been selected for its ability to recognize LpPLA2 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant LpPLA2 (Phe22~Asn441) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ).脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體 Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining on fromalin fixed paraffin- embedded Liver tissue Used in Western Blot ,Sample: Recombinant LpPLA2,Human

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無(wú)標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)多克隆抗體WB, ICC, IHC-P, IHC-F, ELISA