P-選擇素糖蛋白配體1(PSGL1)多克隆抗體 

Polyclonal Antibody to P-Selectin Glycoprotein Ligand 1 (PSGL1)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: PSGL1, Mouse

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.P-選擇素糖蛋白配體1(PSGL1)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant PSGL1 (Ser94~Ser345) expressed in E.coli. Accession No.: RPA985Mu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

GST-tag and its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS -SERATEK

IATTDPTAPG TGGTAVGMLS TDSATQWSLT SVETVQPAST EVETSQPAPM EAETSQPAPM

EAETSQPAPM EAETSQPAPM EADTSQPAPM EAETSQPAPN EAETSKPAPT EAETSKPAPT

EAETTQLPRI QAVKTLFTTS AATEVPSTEP TTMETASTES NESTIFLGPS VTHLPDSGLK

KGLIVTPGNS PAPTLPGSSD LIPVKQCLLI ILILASLATI FLVCTVVLAV RLSRKTHMYP VRNYS

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against PSGL1. It has been

selected for its ability to recognize PSGL1 in immunohistochemical staining and

western blotting.P-選擇素糖蛋白配體1(PSGL1)多克隆抗體

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant PSGL1 (Ser94~Ser345)

disposed in loading buffer.P-選擇素糖蛋白配體1(PSGL1)多克隆抗體

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin- embedded spleen tissue

Used in Western Blot, Sample:

Recombinant PSGL1, Mouse

• 來源多抗制備
• 宿主P-選擇素糖蛋白配體1(PSGL1)多克隆抗體兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無(wú)標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA