**性/通透性增加蛋白(BPI)多克隆抗體 

Polyclonal Antibody Antibody Antibody Antibody to Bactericidal/Permeability Bactericidal/Permeability Bactericidal/Permeability Bactericidal/Permeability Increasing Increasing Increasing Increasing Protein Protein Protein Protein (BPI)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

6th Edition (Revised in March, 2013)

[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: BPI, Human

Clonality: Clonality: Clonality: Clonality: Polyclonal

Host: Rabbit

Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG

Purification: Purification: Purification: Purification: Affinity Chromatography.**性/通透性增加蛋白(BPI)多克隆抗體

Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F

Concentration: Concentration: Concentration: Concentration: 200μg/mL

UOM: 100μg Sample: Recombinant BPI, Human

[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen : Immunogen : Immunogen : Immunogen : Recombinant BPI (Glu140~Th r250 ) exp ressed in E.coli. USCN Accession Accession Accession Accession No.: P91234Hu01

Sequence: Sequence: Sequence: Sequence: The target protein is fused with two N-terminal Tags, His-tag and

GST-tag and its sequence is listed below.

MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL

DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD

VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD

GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ

GPLGSEF- E GMSISADLKL GSNPTSGKPT ITCSSCSSHI NSVHVHISKSKVGWLIQLFH

KKIESALRNK MNSQVCEKVT NSVSSELQPY FQTLPVMTKI DSVAGINYGL VAPPATTAET

[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against BPI. It has been

selected for its ability to recognize BPI in immunohistochemical staining and

western blotting.**性/通透性增加蛋白(BPI)多克隆抗體

[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Optimal working dilutions must be determined by end user.

[ CONTENTS CONTENTS CONTENTS CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY QUALITY QUALITY QUALITY CONTROL ]

Content: Content: Content: Content: The quality control contains recombinant BPI (Asp159~Lys258)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemil umes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.**性/通透性增加蛋白(BPI)多克隆抗體

Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

• 來源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度**性/通透性增加蛋白(BPI)多克隆抗體200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA