表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)多克隆抗體 

Polyclonal Antibody to Surfactant Associated Protein B (SPB)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: SPB, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant SPB (Phe201~Leu381) expressed in E.coli. Accession No.: RPB622Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

GST-tag and its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-FPIPLPYCWL

CRALIKRIQA MIPKGALAVA VAQVCRVVPL VAGGICQCLA ERYSVILLDT LLGRMLPQLV

CR LV L R CSM D DS A GP RS PT G EW LP RDS E C H LCM SV T T QAG NS S EQAIP QA

MLQA CVGSW L DREK CK QFVE QHTPQ L LTLV PRGW DA H T T C QA LGV C GTMS

SPLQCIHSPD L

[ ANTIBODY SPECIFITY ]表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against SPB. It has been

selected for its ability to recognize SPB in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant SPB (Phe201~Leu381)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)多克隆抗體

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin-embedded lung tissue

Used in Western Blot, Sample:

Recombinant SPB, Human

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)多克隆抗體無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA