Smad同源物4(Smad4)多克隆抗體 

Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PRODUCT INFORMATION ]

Immunogen: Smad4, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.Smad同源物4(Smad4)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant Smad4 (Trp323~Asp552) with two N-terminal Tags,

His-tag and T7-tag expressed in E.coli.

Accession No.: RPB925Hu01

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against Smad4. It has been

selected for its ability to recognize Smad4 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.Smad同源物4(Smad4)多克隆抗體

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,

50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant Smad4 (Trp323~Asp552)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL per well when used in enhanced chemilumescent (ECL).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,

BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20

oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.Smad同源物4(Smad4)多克隆抗體

[ IMAGES ]

Used in Western Blot, Sample:

Recombinant Smad4, Human

Used in DAB staining on fromalin fixed

paraffin- embedded skeletal muscle tissue

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀Smad同源物4(Smad4)多克隆抗體液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA