來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
伴刀豆球蛋白A(ConA)多克隆抗體標(biāo)記物無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
伴刀豆球蛋白A(ConA)多克隆抗體Polyclonal Antibody to Concanavalin A (ConA)
Organism Species: General
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: ConA
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
伴刀豆球蛋白A(ConA)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Native Protein ConA. Accession No.: NPC179Ge01
[ RELEVANCE ]
Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally
extracted from the jack-bean, Canavalia ensiformis. It is a member of the legume
lectin family. It binds specifically to certain structures found in various sugars,
glycoproteins, and glycolipids, mainly internal and nonreducing terminal α
-D-mannosyl and α-D-glucosyl groups. ConA is a plant mitogen, and is known
for its ability to stimulate mouse T-cell subsets giving rise to four functionally
distinct T cell populations, including precursors to suppressor T-cell; one subset
of human suppressor T-cells as well is sensitive to ConA. Like most lectins, ConA
is a homotetramer: each sub-unit (26.5KDa, 235 amino-acids, heavily glycated)
binds a metallic atom (usually Mn2+ and a Ca2+).
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against ConA. It has been
selected for its ability to recognize ConA in immunohistochemical staining and
western blotting.
伴刀豆球蛋白A(ConA)多克隆抗體[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.