70kDa熱休克蛋白1B(HSPA1B)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 70kDa熱休克蛋白1B(HSPA1B)多克隆抗體標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
• 70kDa熱休克蛋白1B(HSPA1B)多克隆抗體Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)
  Organism Species: Bos taurus; Bovine (Cattle)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: HSPA1B, Bovine
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200μg/mL
  UOM: 100μg Sample: Recombinant HSPA1B, Bovine
  70kDa熱休克蛋白1B(HSPA1B)多克隆抗體[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant HSPA1B (Met1~Asp641) expressed in E.coli. USCN Accession No.: RPA301Bo01
  Sequence: The target protein is fused with N-terminal His-Tag and its sequence
  is listed below.
  MGHHHHHHSGSEF-MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS
  YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFRVINDGDK
  PKVQVSYKGE TKAFYPEEISSMVLTKMKEI AEAYLGHPVT NAVITVPAYF NDSQRQATKD
  AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK
  ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA
  SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS
  TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
  LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT RDNNLLGRFE
  LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ
  EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW
  LDANTLAEKD EFEHKRKELE QVCNPIISRL YQGAGGPGAG GFGAQGPKGG SGSGPTIEEV
  D
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against HSPA1B. It has been
  selected for its ability to recognize HSPA1B in immunohistochemical staining and
  western blotting.
  70kDa熱休克蛋白1B(HSPA1B)多克隆抗體[ APPLICATIONS ]
  Western blotting: 1:100-400
  Immunocytochemistry in formalin fixed cells: 1:100-500
  Immunohistochemistry in formalin fixed frozen section: 1:100-500
  Immunohistochemistry in paraffin section: 1:50-200
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant HSPA1B (Met1~Asp641)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.