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產(chǎn)品名稱: 胞漿型磷脂酶A2(cPLA2)單克隆抗體
產(chǎn)品型號(hào): cPLA2
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
胞漿型磷脂酶A2(cPLA2)單克隆抗體是針對(duì)cPLA2的小鼠單克隆抗體。在**組織化學(xué)染色和**印跡實(shí)驗(yàn)中能識(shí)別cPLA2。胞漿型磷脂酶A2(cPLA2)單克隆抗體主要用于WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM等多種**學(xué)實(shí)驗(yàn)，一抗，二抗，標(biāo)簽抗體，內(nèi)參抗體、信號(hào)通路抗體、細(xì)胞凋亡抗體等現(xiàn)貨供應(yīng)，獲得高靈敏度和高特異性的一致性結(jié)果的不二之選，產(chǎn)品靈敏度高，可重現(xiàn)性好，相同的名稱，不同的物種，多種規(guī)格供應(yīng),售后完善。
胞漿型磷脂酶A2(cPLA2)單克隆抗體
的詳細(xì)介紹
胞漿型磷脂酶A2(cPLA2)單克隆抗體
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來源單抗制備
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宿主小鼠
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效價(jià)(克隆號(hào))C1
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Ig亞型IgG2b Kappa
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純化方式蛋白A + 蛋白G親和純化
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標(biāo)記物無(wú)標(biāo)記物
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胞漿型磷脂酶A2(cPLA2)單克隆抗體緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度500ug/ml
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且適物種-
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應(yīng)用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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胞漿型磷脂酶A2(cPLA2)單克隆抗體Monoclonal Antibody to Phospholipase A2, Cytosolic (cPLA2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG2b Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: C1
Traits: Liquid
Concentration: 500μg/mL
UOM: 200μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPB624Hu01-Recombinant Phospholipase A2, Cytosolic (cPLA2)
胞漿型磷脂酶A2(cPLA2)單克隆抗體[ APPLICATIONS ]
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant cPLA2 disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.胞漿型磷脂酶A2(cPLA2)單克隆抗體 [ IDENTIFICATION ]
A
Figure 1. Western Blot
A. Sample: Recombinant cPLA2, Human B. Lane1: Human Lung Tissue Lane2: Human PC-3 Cells Lane3: Human Hela Cells Primary Ab: 1μg/mL Rabbit Anti-Human cPLA2 Ab
Second Ab: 1:5000 Dilution of HRP-Linked Rabbit
Anti-Mouse IgG Ab (Catalog: SAA544Mu09)
B
Figure 2. DAB staining on IHC-P Samples： A. Human Kidney Tissue B. Human Pancreas Tissue C. Human Glioma Tissue D. Human Stomach Tissue A B C D
