來源單抗制備
宿主小鼠
效價(克隆號)3#
Ig亞型IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體標(biāo)記物無標(biāo)記物
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度500ug/ml
且適物種-
應(yīng)用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體Monoclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG2b Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: 3#
Traits: Liquid
Concentration: 500μg/mL
UOM: 200μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體 [ IMMUNOGEN ]
Immunogen: RPF878Hu01-Recombinant Secreted Frizzled Related Protein 4
(SFRP4)
[ APPLICATIONS ]
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,
50% glycerol. [ QUALITY CONTROL ]
Content: The quality control contains recombinant SFRP4 disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體 [ IDENTIFICATION ]
A
Figure 1. Western Blot
A. Sample: Recombinant SFRP4, Human B. Lane1: Human Hela Cells Lane2: Human 293T Cells Lane3: Human SKOV3 Cells Lane4: Porcine Heart Tissue Primary Ab: 3μg/mL Rabbit Anti-Human SFRP4 Ab
Second Ab: 1:5000 Dilution of HRP-Linked Rabbit
Anti-Mouse IgG Ab (Catalog: SAA544Mu09) B