細(xì)胞形態(tài)： **樣

**類型： IgG3

ATCC Number： HB-8248?

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: HT 29/36 [SKI 294/HT 29/36]

HT 29/36細(xì)胞Depositors: Sloan-Kettering Institute for Cancer Research

Isotype: IgG3

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human colon adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HT 29/36細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Comments: Animals were immunized with a human colon cancer cell line (HT-29, see ATCC HTB-38).

The antibody reacts with human colon adenocarcinoma cells.

It does not react with frozen sections of normal ***** tissues.

This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers.

Propagation: HT 29/36細(xì)胞ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids and 2% HT, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 90%; DMSO, 10%

References: 3882: Cordon-Cardo C, et al. Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies. HT 29/36細(xì)胞US Patent 4,579,827 dated Apr 1 1986