品系： BALB/c
生長狀態(tài)： 貼壁生長
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 轉(zhuǎn)基因小鼠
細胞形態(tài)： 上皮樣
運輸方式： 凍存運輸
器官來源： 睪丸
年限： 10 days
數(shù)量： 大量
ATCC Number： CRL-2053?
相關(guān)**： 其他**
GC-1 spg細胞Designations: GC-1 spg
Depositors: MC Hofmann
Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus, transgenic for SV40 early region deposited as mouse, transgenic for SV40 early region
Morphology: epithelial
Source: Organ: testis
Strain: BALB/c
Disease: spermatogonia
Cell Type: transformed with pSV3-neo
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Age: 10 days
Gender: male
Comments: GC-1 spg細胞Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance).
The line shows characteristics of a stage between type B spermatogonia and primary spermatocytes.
The cells express two testis specific isoproteins, cytochrome c and lactate dehydrogenase C4.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
GC-1 spg細胞Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23124: GC-1 spg細胞Hofmann MC, et al. Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen. Exp. Cell Res. 201: 417-435, 1992. PubMed: 1322317
