生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 13 weeks gestation
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
器官來源： 結(jié)腸
ATCC Number： CRL-1831?
相關(guān)**： 正常
是否是腫瘤細(xì)胞： 0
物種來源： 人
Designations: FHC
Depositors: DP Chopra
FHC細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 6,9.3
TPOX: 8,11
vWA: 16
Age: 13 weeks gestation
Comments: When using a non-humidified incubator, gassing of cultures with 5% CO2 and plug-seal caps should be used to prevent evaporation of liquid in media and unintentional concentrating of media additives.
Although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected.
Propagation:FHC細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
fetal bovine serum 10%(final conc.)
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
FHC細(xì)胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 4 to 6 X 10(3) viable cell/cm2 is recommended.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days
Medium Renewal: Every 3 to 4 days.
Preservation: Freeze medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 22672: Siddiqui KM, Chopra DP.FHC細(xì)胞 Primary and long term epithelial cell cultures from human fetal normal colonic mucosa. In Vitro 20: 859-868, 1984. PubMed: 6519668
