細(xì)胞類型： SV40轉(zhuǎn)化細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 人
ATCC Number： CRL-1584?
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
器官來源： 胎盤
運輸方式： 凍存運輸
Designations: 3A-sub E [post crisis of 3A(tPA-30-1)]
Depositors: MI Cour
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
3A-sub E細(xì)胞Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: placenta
Cell Type: SV40 transformed
Cellular Products: at 40C the cells produce human chorionic gonadotropin (hCG)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. 3A-sub E細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17
3A-sub E細(xì)胞Comments: This line was established from ATCC CRL-1583 cells that had begun to senesce.
By passage 20 the cells had recovered and appeared capable of unlimited proliferation.
The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, rinse culture with fresh 0.25% trypsin-0.03% EDTA solution and let sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium 3A-sub E細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
