年限： 8 years
ATCC Number： CRL-1942?
相關**： 其他**
細胞形態(tài)： **樣
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
細胞類型： T**細胞
是否是腫瘤細胞： 0
物種來源： 人
Designations: SUP-T1 [VB]
Depositors: SD Smith
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
SUP-T1細胞Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: lymphoblastic leukemia
Cell Type: T lymphoblast;
Cellular Products: immunoglobulin (cytoplasmic)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Antigen Expression: Leu-6 (CD1a) +; Leu-4 (CD3) +; Leu-3 (CD4) +; Leu-1 (CD5) +; Leu-9 (CD7) +; Leu-2a (CD8) +; OKT-10 (CD38) + [23053] [23503] [23504]
Cytogenetic Analysis: 92, XXYY, -2, -2, -9, -9, the following markers are present: 2inv(2*) (p21q11), +2inv(2*), del(2*)(Q21), 2del(4)(q31q35), 2del(5)(p13p14), 2del(6)(q23q27), 2inv(14)(q11q32), t(7;9)(q34;q34), der(9)t(7;9)
Age: 8 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from malignant cells collected from the malignant pleural effusion of an 8 year old child with T-LL.
The cells express multiple T lineage markers.
The cells are E - rosette negative and beta 2 microglobulin positive.
SUP-T1細胞The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) and Tac (CD25).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp5 viable cells/ml and maintain between 2 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22618: Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650
22628: Baer R, et al.SUP-T1細胞 Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328
22629: Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364
23053: Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672
23503: Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800
23504: Lifson JD, et al. SUP-T1細胞AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463
