年限： 53 years
ATCC Number： HTB-181?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
數(shù)量： 大量
器官來(lái)源： 肺
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞形態(tài)： 上皮樣
Designations: NCI-H820 [H820]
Depositors: AF Gazdar, JD Minna
NCI-H820細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: papillary adenocarcinoma
Derived from metastatic site: lymph node
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Isolation: Isolation date: 1984
Tumorigenic: YES
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 9,11
D7S820: 10,13
THO1: 8
NCI-H820細(xì)胞TPOX: 12
vWA: 18
Cytogenetic Analysis: Near triploid; modal number = 69; range = 46 to 74; there were 25 to 35 marker chromosomes per metaphase; the i(7p), t(6p;??) and ?i(8q) were the few identifiable markers. One B-like chromosome (Bq+) had a length comparable to N1; another B size chromosome had an interstitial HSR segment. There were 1 to 2 structurally normal X chromosomes, and two or more Y chromosomes were detected in the QM stained metaphases.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Age: 53 years
Gender: male
Ethnicity: Caucasian
Comments: The cell line expresses three surfactant associated proteins (SP-A constitutively, and SP-B and SP-C after dexamethasone induction).
Electron microscopy shows intracytoplasmic multilamellar bodies suggestive of Type II pneumocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
strong>Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: NCI-H820細(xì)胞Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37.0°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium renewal: Every 2 to 3 days
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium NCI-H820細(xì)胞(without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Phosphate-buffered saline: ATCC 30-2200
Cell culture tested DMSO: ATCC 4-X
References: 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
23081: Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953
