ATCC Number： HB-229?

細(xì)胞形態(tài)： **樣

**類型： IgM

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

Designations: MD2

Depositors: RV Rouse

Isotype: IgM

MD2細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus MD2細(xì)胞(myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium

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Comments: Animals were immunized with mouse thymic stromal tissue.

Spleen cells were fused with Sp2/0 myeloma cells.

MD2細(xì)胞In the mouse thymus, MD2 is specific for dendritic cells in the medulla.

The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.

It also reacts with epithelial elements in a variety of mouse tissues.

Propagation: ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 58334: Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. MD2細(xì)胞PubMed: 2461413