數(shù)量： 大量
生長狀態(tài)： 懸浮生長
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： HB-8191?
細(xì)胞形態(tài)： **樣
**類型： IgM
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
Designations: alpha-ACE 3.1.1 [ACE 3.1.1, a-ACE 3.1.1]
Depositors: Wisconsin Alumni Res. Fndn.
Isotype: IgM
alpha-ACE 3.1.1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against angiotensin converting enzyme (ACE)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
alpha-ACE 3.1.1細(xì)胞Tumorigenic: Yes
Comments: Animals were immunized with a crude preparation of rat lung ACE with incomplete Freund's adjuvant.
Spleen cells were fused with P3-NSI/1-Ag4/1 myeloma cells.
The antibody reacts with purified ACE and endothelial cells of mouse, bovine and human origin.
The antibody can be used to isolate endothelial cells from the above species.
Propagation: ATCC complete growth medium: alpha-ACE 3.1.1細(xì)胞Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.005 mM 2-mercaptoethanol, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1649: Auerbach R, et al. Monoclonal antibody against angiotensin-converting enzyme: Its use as a marker for murine, bovine, and human endothelial cells. Proc. Natl. Acad. Sci. USA 79: 7891-7895, 1982. PubMed: 6296854
24374: alpha-ACE 3.1.1細(xì)胞Auerbach R. Monoclonal antibody to angiotension-converting enzyme and methods of preparing and using same. US Patent 4,582,790 dated Apr 15 1986
