生長狀態(tài)： 混合型生長

品系： C57BL

細(xì)胞形態(tài)： 其他

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： CRL-1642?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

器官來源： 肺

相關(guān)**： 其他**

Designations: LL/2 (LLC1)

LL/2 (LLC1)細(xì)胞Depositors: JS Bertram

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Mus musculus

Morphology: rounded - loosely attached or floating

Source: Strain: C57BL

Organ: lung

Disease: Lewis lung carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. LL/2 (LLC1)細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.

Applications: Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.

Tested and found negative for ectromelia virus (mousepox).

The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.

This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.

Tumorigenic: Yes

Antigen Expression: H-2b

Comments: This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.

Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.

The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.

The cells are reported to be highly tumorigenic, but weakly metastatic in mice.

LL/2 (LLC1)細(xì)胞The cells form multilayers in flasks without actually becoming confluent.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 21 hrs

Related Products: Recommended mediumLL/2 (LLC1)細(xì)胞 (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1091: Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139

45212: Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207