數(shù)量： 大量

ATCC Number： HTB-52?

相關(guān)**： 腺癌

是否是腫瘤細(xì)胞： 0

物種來源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 肝

細(xì)胞形態(tài)： 上皮樣

組織來源： ascites

年限： 52 years

Designations: SK-HEP-1

SK-HEP-1細(xì)胞Depositors: G Trempe, LJ Old

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: liver

Tissue: ascites

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Isolation: Isolation date: 1971

Applications: transfection host (Roche Transfection Reagents)

SK-HEP-1細(xì)胞Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 8,12

D16S539: 12

D5S818: 10,13

D7S820: 8,11

THO1: 7,9

TPOX: 9

vWA: 14,17

Cytogenetic Analysis: The cell line is aneuploid human (XX), with chromosome counts in the hypotriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N7, N12, and N17 tend to be over-represented in some metaphases. Eight markers are identified: del(1)(q21), der(1)t(1;2)(p36;q21), ?tdic(1;12)(p36;q24), ampl.t(4pter--->4q11::HSR::4q12--->4q24::HSR?::4q26--->4q35::2q15--->2pter), 13p+, 13p++, 14p+, t(14q15q), iso(14q), 19q+, del(3)(p14p24), 15p+.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1-2

SK-HEP-1細(xì)胞PGM1, 2

PGM3, 1

Age: 52 years

Gender: male

Ethnicity: Caucasian

Comments: The SK-HEP-1 line has been identified as being of endothelial origin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOComplete growth medium supplemented with 5% (v/v) DMSO

SK-HEP-1細(xì)胞Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

24396: Heffelfinger SC, et al. SK HEP-1: a human cell line of endothelial origin. In Vitro Cell. Dev. Biol. 28A: 136-148, 1992. PubMed: 1371504

56315: Gucev ZS, et al. Evidence for insulin-like growth factor (IGF)-independent transcriptional regulation of IGF binding protein-3 by growth hormone in SKHEP-1 human hepatocarcinoma cells.SK-HEP-1細(xì)胞 Endocrinology 138: 1464-1470, 1997. PubMed: 9075703

58075: Turner BM, Turner VS. Secretion of alpha1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids. Somatic Cell Genet. 6: 1-14, 1980. PubMed: 6245472