數(shù)量： 大量

ATCC Number： CRL-1582?

相關(guān)**： 白血病

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： T**細(xì)胞

生長狀態(tài)： 懸浮生長

是否是腫瘤細(xì)胞： 0

物種來源： 人

細(xì)胞形態(tài)： **樣

年限： 19 years

Designations: MOLT-4

Depositors: J Minowada

MOLT-4細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: acute lymphoblastic leukemia

Cell Type: T lymphoblast;

Cellular Products: high levels of terminal deoxynucleotidyl transferase (TdT) are produced

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

MOLT-4細(xì)胞Antigen Expression: CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)

DNA Profile (STR): Amelogenin: XY

CSF1PO: 11, 12, 13

D13S317: 12, 13

D16S539: 11, 14

D5S818: 12

D7S820: 8, 10, 11

THO1: 6, 8

TPOX: 8

vWA: 17, 18

Cytogenetic Analysis: This is a human cell line with the hypertetraploid chromosome number. The modal chromosome number was 95 occurring in 24% of cells. The rate of cells with higher ploidies was 0.8%. The 6q- and t(7;7) were paired and also common to all cells. The i(17q) was single and occurred only in some cells. MOLT-4細(xì)胞Most normal chromosomes had four copies per cell except for the paired N7, X and Y, and two to three copies for N17.

Age: 19 years

Gender: male

Comments: The line was established from cells taken from a patient in relapse.

The patient had received prior multidrug chemotherapy.

There is a G -> A mutation at codon 248 of the p53 gene.

P53 is not expressed.

The cells do not produce immunoglobulin or Epstein-Barr virus.

MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC CRL-1552).

The line was originally contaminated with mycoplasma but has been cured with antibiotics.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10(5) cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

MOLT-4細(xì)胞Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-1552

References: 22524: Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231

22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523

22735: Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897

23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

23337: Sandstrom PA, Buttke TM. MOLT-4細(xì)胞Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323