是否是腫瘤細胞： 0
物種來源： 倉鼠
器官來源： 肺
ATCC Number： CCL-93?
細胞形態(tài)： 成纖維樣
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
數(shù)量： 大量
Designations: V79-4
Depositors: EH Chu
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
V79-4細胞Organism: Cricetulus griseus
Morphology: fibroblast
Source: Organ: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: modal number = 22; range = 20 to 23, Pseudodiploid. The rate of higher ploidies was 4%. Twelve to fourteen marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6,?), 7q+ and seven to eight other small markers., Normal N2 and N3 were paired; N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell.
Gender: male
Comments: This cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V.
V79-4細胞The line was renamed V79 by Elkind in 1958,and was used in studies of X-ray induced damage and repair processes.
In 1966, E.H.Y. Chu obtained the line from W. Sinclair, and isolated the V79-4 clone.
The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours.
A culture at passage 7 after cloning was submitted to the ATCC in July 1988.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:14 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium V79-4細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1657
References: 21877: . Molecular cell genetics. New York: Wiley; 1985.
22642: Chu EH, et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-377, 1969. PubMed: 5392645
23252: Chu EH, Malling HV. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968. PubMed: 5249812
26264: . . Nature 184: 1293-1295, 1959.
26265: Ford DK, Yerganian G. Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Natl. Cancer Inst. 21: 393-425, 1958. PubMed: 13576097
26275: . Molecular cell genetics. New York: Wiley; 1985.
32373: Goodrum FD, et al. V79-4細胞Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
