數(shù)量： 大量

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-2755?

相關(guān)**： 其他**

運輸方式： 凍存運輸

品系： BALB/cCrgl

細胞形態(tài)： 上皮樣

細胞類型： 上皮細胞

器官來源： 其他

是否是腫瘤細胞： 0

物種來源： 小鼠

Designations: EMT6

Depositors: S Rockwell

Biosafety Level: 1

EMT6細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: breast

Strain: BALB/cCrgl

Disease: mammary carcinoma

Cell Type: epithelial

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Isolation: Isolation date: 1971

Tumorigenic: Yes

Gender: female

Comments: EMT6 was established from a transplantable murine mammary carcinoma that arose in a BALB/cCRGL mouse after implantation of a hyperplastic mammary alveolar nodule. EMT6細胞The resulting tumor line (named KHJJ) was propagated in BALB/cKa mice and adapted to tissue culture after the 25th animal passage, and the cell line was named EMT. EMT6 is a clonal isolate of EMT isolated in 1971 at Stanford University. The EMT6 cell line can be grown either in animals as a tumor or in tissue culture. Cells derived from tumors have a reported in vitro plating efficiency of 30%. Cell grown in tissue culture reportedly have a plating efficiency of 70%.

Propagation: ATCC complete growth medium: Waymouth's MB 752/1 Medium with 2mM L-glutamine, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

EMT6細胞Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 61342: Rockwell SC, et al. Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative. J. Natl. Cancer Inst. 49: 735-749, 1972. PubMed: 4647494

61343: Palom Y, et al. Structure of adduct X, the last unknown of the six major DNA adducts of mitomycin C formed in EMT6 mouse mammary tumor cells. Chem. Res. Toxicol. 13: 479-488, 2000. PubMed: 10858321

61344: Collingridge DR, Rockwell S. Pentoxifylline improves the oxygenation and radiation response of BA1112 rat rhabdomyosarcomas and EMT6 mouse mammary carcinomas. Int. J. Cancer 90: 256-264, 2000. PubMed: 11091349

61345: Rockwell S, Kelley M. RSR13, a synthetic allosteric modifier of hemoglobin, as an adjunct to radiotherapy: preliminary studies with EMT6 cells and tumors and normal tissues in mice. Radiat. Oncol.EMT6細胞 Investig. 6: 199-208, 1998. PubMed: 9822166

61346: Rockwell S. In vivo-in vitro tumor systems: new models for studying the response of tumors to therapy. Lab. Anim. Sci. 27: 831-851, 1977. PubMed: 338981

70368: Rockwell STumor-Cell SurvivalIn: Rockwell STumor models in cancer researchTotowa, New JerseyHumana Press Inc.617-631.