ATCC Number： CRL-2404?

運輸方式： 凍存運輸

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 人

生長狀態(tài)： 貼壁生長

年限： 65 year old

器官來源： 皮膚

細胞形態(tài)： 上皮樣

數(shù)量： 大量

Designations: HEK001

HEK001細胞Depositors: PB Sugerman, M Bigby

Biosafety Level: 2 [Cells contain human papilloma viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial

Source: Organ: skin

Cell Type: keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transformed

Cellular Products: keratin

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Isolation: Isolation date: 1996

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 8

D16S539: 12,13

HEK001細胞D5S818: 10,13

D7S820: 12,13

THO1: 6,9

TPOX: 8,9

vWA: 14

Age: 65 year old

Gender: male

Comments: HEK001 cells express keratin 14 but not keratin 10 suggesting that they are proliferating basal-type keratinocytes.

Propagation: ATCC complete growth medium: Keratinocyte-Serum Free medium (GIBCO-BRL 17005-042) with 5 ng/ml human recombinant EGF and 2mM L-glutamine (without bovine pituitary extract and without serum)

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

HEK001細胞Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).

Add 2.0 to 3.0 ml of 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium with 10% FBS added and aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 10% FBS and 5% DMSO

Storage temperature: HEK001細胞liquid nitrogen vapor phase

Related Products: Cell culture tested DMSO:ATCC 4-X

References: 48305: Sugerman PB, Bigby M. Preliminary functional analysis of human epidermal T cells. Arch. Dermatol. Res. 292: 9-15, 2000. PubMed: 10664009