QM7細(xì)胞

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-1962?

相關(guān)**： 纖維肉瘤

年限： 1 to 3 weeks

細(xì)胞形態(tài)： 成纖維樣

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 其他

組織來源： muscle

Designations: QM7 (Quail muscle clone 7)

Depositors: PB Antin

QM7細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Coturnix coturnix japonica

Morphology: fibroblast

Source: Tissue: muscle

Disease: fibrosarcoma

Cell Type: myoblast myoblast; chemically induced

Cellular Products: QM7細(xì)胞desmin; cardiac troponin T; cardiac troponin C; skeletal troponin T; skeletal troponin I; alpha tropomyosin; muscle creatine kinase; myosin light chain 2; myosin heavy chain (ventricular form)

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Age: 1 to 3 weeks

Comments: The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. from a tumor that developed in a bird treated with methylcholanthrene.

QM7 is a serum inducible myogenic cell line.

QM7細(xì)胞The cells replicate as myoblasts in medium containing serum.

When switched to medium without serum, the cells cease dividing and fuse to form large multinucleated myotubes.

In the myotube state, the cells express muscle specific proteins such as desmin, cardiac troponin T, cardiac troponin C, skeletal troponin T, skeletal troponin I, alpha tropomyosin.

Mucle creatine kinase, myosin light chain 2 and a ventricular form of myosin heavy chain.

They do not express any known form of alpha actin.

QM7 cells transfect with high efficiency, and are useful for studying many aspects of muscle differentiation and gene expression.

The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.

To prevent loss of myoblastic cell, cultures should be subcultured before they become confluent and the line should be recloned periodically with selection for myoblastic cells.

Propagation: ATCC complete growth medium: Medium 199 with Earle's BSS, 80%; tryptose phosphate broth, 10%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent. Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin solution. Incubate the flask at room temperature until the cells detach (about 5 minutes).QM7細(xì)胞 Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

References: 23002: Antin PB, Ordahl CP. Isolation and characterization of an avian myogenic cell line. Dev. Biol. 143: 111-121, 1991. PubMed: 1985013

70419: Rong S, Sheppard MG. Processes for preparation of Marek's disease virus using continuous avian cell lines. US Patent 6,410,297 dated Jun 25 2002