BEAS-2B細胞

ATCC Number： CRL-9609?

相關**： 正常

細胞形態(tài)： 上皮樣

細胞類型： 其他細胞類型

器官來源： 肺

是否是腫瘤細胞： 0

物種來源： 人

數量： 大量

運輸方式： 凍存運輸

組織來源： bronchus

生長狀態(tài)： 貼壁生長

BEAS-2B細胞Designations: BEAS-2B

Depositors: The United States of America

Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Tissue: bronchus

Disease: normal

Cell Type: epithelialvirus transformed

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Applications: transfection host

Tumorigenic: No

DNA Profile (STR): Amelogenin: XY

CSF1PO: 9, 12

BEAS-2B細胞D13S317: 13

D16S539: 12

D5S818: 12,13

D7S820: 10, 13

THO1: 7, 9.3

TPOX: 6, 11

vWA: 17, 18

Comments: Epithelial cells were isolated from normal human bronchial epithelium obtained from autopsy of non-cancerous individuals.

The cells were infected with an adenovirus 12-SV40 virus hybrid (Ad12SV40) and cloned.

The cells retain the ability to undergo squamous differentiation in response to serum, and can be used to screen chemical and biological agents for ability to induce or affect differentiation and/or carcinogenesis.

The cells stain positively for keratins and SV40 T antigen.

Propagation: ATCC complete growth medium: BEAS-2B細胞The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit. Note: Do not filter complete medium.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The flasks used should be precoated with with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01 mg/ml bovine serum albumin dissolved in BEBM medium .

Subculturing: Protocol:

Remove and discard culture medium.

Add 2.0 to 3.0 ml of 0.25% Trypsin - 0.53mM EDTA solution containing 0.5% polyvinylpyrrolidone (PVP) to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 10 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh growth medium. Inoculate new flasks at 1500 to 3000 cells per sq. cm. The culture flasks used should be pre-coated with a mixture of 0.01mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01mg/ml bovine serum albumin dissolved in BEBM medium (see reference below).

Place culture flasks in incubators at 37C.

Interval: Subcultured before reaching confluence.

Medium Renewal: Every 2 to 3 days

Preservation: BEAS-2B細胞Freeze medium: Complete growth medium supplemented with 1% PVP and 7.5% DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989

22301: Lechner JF, LaVeck MA. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985.

30067: Sakamoto O, et al. Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. J. Clin. Invest. 99: 701-709, 1997. PubMed: 9045873