PK(15)細(xì)胞

年限： *****

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： CCL-33?

相關(guān)**： 正常

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 豬

器官來(lái)源： 腎臟

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: PK(15)

Depositors: Cutter Laboratories, Inc.

PK(15)細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Sus scrofa

Morphology: epithelial

Source: Organ: kidney

Disease: normal

Cellular Products: plasminogen activator; keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Virus Resistance: poliovirus 2

Age: *****

Comments: PK(15)細(xì)胞The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC .

The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses.

The cell line harbors an endogenous C-type retrovirus.

The cells are positive for porcine circovirus (PCV) antigens.

The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Rinse the cell sheet 2 times with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. PK(15)細(xì)胞Add fresh medium, aspirate and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22988: Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830

26184: Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860

26185: Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256

53398: Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097

53399: Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060

56104: Todaro GJ, et al.PK(15)細(xì)胞 Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403

105588: James A House, personal communication