7-34-1細(xì)胞
ATCC Number： CRL-1945?
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
**類型： IgG2a
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
Designations: 7-34-1
Depositors: CM Warner
Isotype: IgG2a
Biosafety Level: 1
7-34-1細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 7-34-1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with peripheral blood mononuclear cells (PMNC) from SLA c haplotype miniature swine.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific.
It does not cross-react with PMNC from humans, sheep, goats or mice, but does cross-react with cells from commercial varieties of pigs.
Propagation: ATCC complete growth medium: 7-34-1細(xì)胞The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22727: Lie WR, et al. 7-34-1細(xì)胞Preparation and characterization of murine monoclonal antibodies to swine lymphocyte antigens. Immunology 64: 599-605, 1988. PubMed: 2459049
