LLC-PK1細(xì)胞

器官來源： 腎臟

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

品系： Hampshire

年限： 3 to 4 weeks

是否是腫瘤細(xì)胞： 0

物種來源： 豬

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CL-101?

相關(guān)**： 正常

Designations: LLC-PK1

Depositors: Eli Lilly & Co.

LLC-PK1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Sus scrofa

Morphology: epithelial

Source: Organ: kidney

Strain: Hampshire

Disease: normal

Cellular Products: plasminogen activator

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 3 to 4 weeks

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L to 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Twice per week

LLC-PK1細(xì)胞Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: culture medium 95%; DMSO, 5%

Related Products:LLC-PK1細(xì)胞 recommended serum:ATCC 30-2020

formerly distributed as:ATCC CRL-1392

References: 3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975

22659: Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262

28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, LLC-PK1細(xì)胞renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321