R1.G1細(xì)胞
細(xì)胞類型: T**細(xì)胞
是否是腫瘤細(xì)胞: 0
物種來(lái)源: 小鼠
品系: C58/J
數(shù)量: 大量
運(yùn)輸方式: 凍存運(yùn)輸
器官來(lái)源: 胸腺
ATCC Number: TIB-44?
相關(guān)**: **瘤
細(xì)胞形態(tài): **樣
生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)
Designations: R1.G1
Depositors: R Hyman
R1.G1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Organ: thymus
Strain: C58/J
Disease: lymphoma
Cell Type: T lymphocyte;
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Antigen Expression: H-2k; Thy-1.2; TL
Comments: This line was derived from R1.1 (ATCC TIB-42).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22247: R1.G1細(xì)胞Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.