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產(chǎn)品資料

R1.G1細(xì)胞

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產(chǎn)品名稱: R1.G1細(xì)胞
產(chǎn)品型號(hào): R1.G1
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

R1.G1細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。R1.G1細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


R1.G1細(xì)胞  的詳細(xì)介紹

R1.G1細(xì)胞

細(xì)胞類型: T**細(xì)胞

是否是腫瘤細(xì)胞: 0

物種來(lái)源: 小鼠

品系: C58/J

數(shù)量: 大量

運(yùn)輸方式: 凍存運(yùn)輸

器官來(lái)源: 胸腺

ATCC Number: TIB-44?

相關(guān)**: **瘤

細(xì)胞形態(tài): **樣

生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)

Designations: R1.G1

Depositors: R Hyman

R1.G1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast


Source: Organ: thymus

Strain: C58/J

Disease: lymphoma

Cell Type: T lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. R1.G1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2k; Thy-1.2; TL

Comments: This line was derived from R1.1 (ATCC TIB-42).

The cells are resistant to 0.1 mM 6-thioguanine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22247: R1.G1細(xì)胞Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

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